Diosmin has been identified as having antioxidant and cytoprotective action in several experiments. In the kidney transplant, Ischemia-reperfusion injury is still a major problem, and the inflammatory response to ischemia-reperfusion wound exacerbates the resulting renal injury. The current study examined whether Diosmin has randomized effects on ischemia-reperfusion-induced acute kidney injury in rats. In male C57BL/6 mice, renal ischemia-reperfusion injury was induced by bilateral renal pedicle occlusion over a duration of 30 minutes, followed by 48 hours of reperfusion. Diosmin (25 mg/kg oral) was given 4 days prior to injury to ischemia-reperfusion. The saline with phosphate buffer was used as controls. Blood and renal tissues were assessed at 48 hours after the injury to ischemia. Diosmin therapy has significantly reduced blood urea, urinary creatinine and tubular kidney damage (P ˂ .05). Western blot revealed that the amount of hemum oxygenase-1 and B-cell lymphoma-2 was increased significantly by diosmin at 48 hours of injury and the level of inducible nitric oxide synthase attenuated. Diosmin thereby enhances acute renal ischemic reperfusion damage by reducing inflammation and apoptosis.