Objective: Total of 46 patients recruited as per inclusion criteria.ICU patients who were >18 years with radiographic contrast for diagnostic or interventional computed tomography (CT scan), were included. After ethical approval, samples of 5 ml blood and 5 ml urine were collected before contrast exposure and at 4 h, 24 h, and 48 h after contrast exposure. NGAL and Cystatin C assay was done by ELISA, and urinary levels were normalized as per urine creatinine (UCr) values for each sample.CI-AKI is defined as a rise in SCr of ≥0.3 mg/dl within 48 hrs. Methods: Median age was 36 yrs and 13 (43%) were male. On day of inclusion, median SOFA score was 3; 16% In patients having CI-AKI, mean values changes from pre-contrast to at 4 h, 24 h and 48 h after contrast are presented..Kinetics of plasma (P) and urine (U) NGAL and Cystatin C levels (Mean±SD) with p value among patients having CI-AKI P NGAL (ng/ml), Before Contrast(BC)( 708.5±201.76) ,04hrC(851.5±332.05,p=0.07) ,24hrC(1093.25±225.03,p=0.02),48hrC(788±323.4,p=0.21),UNGAL (ng/mg of U Cr)BC(67.63±48.09) ,04hrC(39.69±19.79,p=0.07) ,24hrC(101.97±90,p=0.12) ,48hrC(59.87±56.85,p=0.73) ,P Cystatin C (ng/ml)  BC(4698.85±574.71),04hrC(4704.57±1144.87,p=0.02),24hrC(4428.85±1135.73,p=0.03),48hrC(4288.85±435.8,p=0.17),U Cystatin C (ng/mg of UCr) BC(3 46.06±224.7),04hrC(219.66±72.18,p=0.91),24hrC (470.21±536.28,p=0.99),48hrC(633.61±811.77,p=0.23). Results: ROC curve analysis during pre-contrast exposure:  NGAL, and Cystatin C), both plasma and urine level AUC was significantly higher in patients who develop CI-AKI  and Post-contrast exposure Plasma levels AUC significantly higher than Urine levels. Conclusions: Objective: - Intravenous administration of radiocontrast media is referred to as contrast-induced kidney injury (CI-AKI).CI-AKI is described as the third most common cause of new AKI in hospitalized patients. The occurrence of CI-AKI is reported up to 55% in these high-risk patients.: NGAL (Neutrophil gelatinase-associated Lipocalin)and Cystatin C have been found an early and sensitive marker of acute kidney injury (AKI). Objective: To evaluate biomarkers in plasma (P) and urine (U) after intravenous contrast in adult ICU patients. Methods: Total of 46 patients recruited as per inclusion criteria.ICU patients who were >18 years with radiographic contrast for diagnostic or interventional computed tomography (CT scan), were included. After ethical approval, samples of 5 ml blood and 5 ml urine were collected before contrast exposure and at 4 h, 24 h, and 48 h after contrast exposure. NGAL and Cystatin C assay was done by ELISA, and urinary levels were normalized as per urine creatinine (UCr) values for each sample.CI-AKI is defined as a rise in SCr of ≥0.3 mg/dl within 48 hrs. Results: Median age was 36 yrs and 13 (43%) were male. On day of inclusion, median SOFA score was 3; 16% In patients having CI-AKI, mean values changes from pre-contrast to at 4 h, 24 h and 48 h after contrast are presented..Kinetics of plasma (P) and urine (U) NGAL and Cystatin C levels (Mean±SD) with p value among patients having CI-AKI P NGAL (ng/ml), Before Contrast(BC)( 708.5±201.76) ,04hrC(851.5±332.05,p=0.07) ,24hrC(1093.25±225.03,p=0.02),48hrC(788±323.4,p=0.21),UNGAL (ng/mg of U Cr)BC(67.63±48.09) ,04hrC(39.69±19.79,p=0.07) ,24hrC(101.97±90,p=0.12) ,48hrC(59.87±56.85,p=0.73) ,P Cystatin C (ng/ml)  BC(4698.85±574.71),04hrC(4704.57±1144.87,p=0.02),24hrC(4428.85±1135.73,p=0.03),48hrC(4288.85±435.8,p=0.17),U Cystatin C (ng/mg of UCr) BC(3 46.06±224.7),04hrC(219.66±72.18,p=0.91),24hrC (470.21±536.28,p=0.99),48hrC(633.61±811.77,p=0.23). Conclusions: ROC curve analysis during pre-contrast exposure:  NGAL, and Cystatin C), both plasma and urine level AUC was significantly higher in patients who develop CI-AKI  and Post-contrast exposure Plasma levels AUC significantly higher than Urine levels.