Objectives: Phytolaccaceae is a poisonous plant that is associated with cardiac and gastrointestinal toxicity. However, there are limited data about acute kidney injury (AKI) in patients with Phytolaccaceae ingestion. We conducted this study to investigate the clinical characteristics of AKI by Phytolaccaceae overdose. Methods: From 2010 to 2021, 13 patients with Phytolaccaceae ingestion were enrolled in this study. The incidence, clinical characteristics, and severity of AKI were compared between AKI and non-AKI groups according to the RIFLE classification. Results: Of the 13 patients, 7 patients experienced AKI during hospitalization period. Compared to patients without AKI, the eGFR was lower in the AKI group (56 ± 12 ml/min/1.73m2 vs. 76 ± 24 ml/min/1.73m2, p<0.05) on admission. The AKI group had a significantly longer hospital stay (9.6 ± 6.2 vs 6.2 ± 3.1 days, p<0.001). The overall AKI incidence was 53.8%, of which 38.4% and 15.4% cases were classified as risk and injury, respectively. All patients recovered baseline renal function without renal replacement therapy following antibiotics therapy and supportive care.  Conclusions: The incidence of AKI in patient with Phytolaccaceae ingestion is 53.8%. Most patients with AKI had a mild type of AKI during admission period. In cases of AKI patients with Phytolaccaceae ingestion, supportive care including hydration is very important.