Objectives: Ascites in End Stage Kidney Disease (ESKD) patients indicates worse outcomes. Complex relationship of gut microbiome and its Short Chain Fatty Acid (SCFA) metabolites on Chronic Kidney Disease (CKD) are reported, however its relation to hemodialysis (HD) patients with ascites is rarely examine. This research aimed to investigate the association of gut microbiome SCFA and ascites in routine hemodialysis patients. Methods: This was a single center observational study of adult ESKD patients in routine twice weekly HD. Subjects were divided into ascites group and without. Social demographic, physical examination, blood laboratory and fecal samples were taken. Inclusion criteria were patients ≥17 years old on twice weekly HD while exclusion criteria are Serum albumin <2,5 g/dL, consumed antibiotics for 1 week prior and refuse to participate. Bivariate and multivariate analysis were done. Normally distributed data were tested by independent t-test and Mann-Whitney test used for non-normally distributed data. Multivariate analysis done by logistic regression.  Results: Twenty-eight subjects were enrolled, 17 had ascites. More females had ascites (19 vs 9), mean age was 42 years with no significant difference between groups, as well as mean blood pressure and hemoglobin levels. Ascites group had higher erythrocyte sedimentation rate (58.18 VS 47.10 mm/hour) and lower albumin (3.75 vs 4.03 g/dL). Higher amount of Acetate (13.08 vs 7.98 mg/dL) and Valerate (0.33 vs 0.19 mg/dl) SCFA are observed in ascites group, while Propionate (17.71 vs 20.26 mg/dL) and Butirate (2.36 vs 2.37 mg/dL) SCFA are lower or comparable. Bivariate analysis showed that Valerate SCFA significantly higher in the ascites group (p=0.016). Multivariate analysis suggests weak association between Propionate (p=0,053) and Valerate (p=0.087) SCFA with ascites in HD patients.  Conclusions: Low Propionate and high Valerate levels tend to have association with ascites in HD patients.