Objectives: Tribulus terrestris in one of leading medicinal plant which is used in treatment of varius kidney disorder including stone by various ayurvedic practitioner. Based on the literature survey, two more medicinal plants Terminalia chebula and Tinsopora cordifolia were selected, which were claimed in traditional medicine to dissolve renal stones. So the present study was undertaken to investigate the combine synergestic antiurolithiatic effect of Tribulus terrestris Linn (Gokhru) with aqueous extract of Terminalia chebula and Tinsopora cordifolia. Methods: Renal calculi were induced in rats by administration of 0.75% Ethylene glycol in drinking water and co‑treated orally with standard drug, Cystone (750 mg/kg) (Group I), with combined aqueous extracts (200 mg of Tribulus terrestris Aq. Extract with 100 mg Terminalia chebula and Tinsopora cordifolia Aq. extract) (Group II) in doses of 500 mg/kg and with aqueous extract of Tribulus terrestris alone (Group III) in doses of 500 mg/kg for 21 days. Weekly body weights were recorded. On day 22, urolithiasis was confrmed by assessing the urinary parameters (urine volume, pH, uric acid, calcium, phosphorus, oxalate, magnesium and creatinine clearance), serum biochemical parameters (creatinine, uric acid, urea, calcium, phosphorus and magnesium), oxidative stress parameters and histology of kidney. Results: Treatment with Combined extracts (Group II) attenuated the EG‑induced decrease significantly in body weight and elevation in urinary parameters (uric acid, calcium, phosphorus and oxalate) and serum biochemical parameters (creatinine, uric acid, urea, calcium, phosphorus and magnesium) as compared to single extract of Gokhru (Group III). Combine extract treatment also reversed EG‑induced decrease in urine volume, pH, magnesium and creatinine clearance, oxidative and histological damages in kidneys Conclusions: Combined aqueous extracts of Tribulus terrestris with Terminalia chebula and Tinsopora cordifolia showed preventive effect against renal calculi formation and validates its ethnomedicinal use in urinary disorders. It further supports its therapeutic potential for the treatment of urinary calculi.