Objectives: T50 is a novel serum-based marker that assesses the propensity of calcification in serum. Shorter T50 indicates greater propensity to calcify and it has been associated to cardiovascular disease (CVD) and mortality among patients with chronic kidney disease (CKD). The association factors of T50 and the correlation between T50 and bone mineral density (BMD) are unknown in hemodialysis (HD) patients. Methods: We performed a cross-sectional study selecting 184 HD patients from Gachon University Gil Medical Center. We categorized individuals into tertiles of T50 and compared demographics and disease indicators across tertiles. We utilized linear regression to evaluate the cross-sectional association between T50 and hip and spine BMD in multivariable models. Results: Older age and the prevalence of CVD and peripheral arterial disease, contrary to reported in patients with CKD, were not associated with T50. Mineral and inflammatory parameters including serum phosphate and albumin were associated with T50. We found a weak association between T50 and BMD in the total hip area in unadjusted or multivariate-adjusted models but did not find a statistically significant association between femoral neck or spinal BMD. Conclusions: T50, a novel indicator of serum calcification propensity, was strongly associated with mineral and inflammatory parameters but not conclusively established association with BMD in HD patients. Future larger studies should determine if T50 may give insights to BMD in this population above and beyond traditional risk factors.