Objectives: Diabetic nephropathy (DN) is a condition that occurs in diabetics and is characterized by structural and functional alterations in the kidneys. The goal of this study was to see how the flavonoid-rich fraction of Alstonia scholaris (FRFAS) affected DN modulation via antioxidant and anti-inflammatory properties.Diabetic nephropathy (DN) is a condition that occurs in diabetics and is characterized by structural and functional alterations in the kidneys. The goal of this study was to see how the flavonoid-rich fraction of Alstonia scholaris (FRFAS) affected DN modulation via antioxidant and anti-inflammatory properties. Methods: Methanol was used to extract the known amount of A scholaris leaves powder. To get FRFAS, methanolic extract was fractionated serially with n-Hexane, chloroform, ethyl acetate, n-butanol, and water. An in vitro experiment verified the presence of flavonoid. A single i.v. injection of streptozoticin (45 mg/kg) was used to create an experimental diabetic rat model. The FRFAS was given orally in doses of 20, 40, and 80 mg/kg/day. Serum and urine chemistry were used to assess renal function. Biochemical assays were used to determine metabolic profiles, renal enzymes, and oxidative stress. ELISA was used to detect cytokines. Histopathologic studies were used to evaluate the pathological alterations. Results: Diabetic rats had renal impairment after receiving STZ, as demonstrated by decreased creatinine clearance, increased blood urea nitrogen and proteinuria, significant increases in lipid peroxidation, decreased anti-oxidant enzyme, and changed cytokine levels. FRFAS effectively restored all of these defects in a dose-dependent manner. In comparison to diabetic rats, FRFAS also repaired the cellular architecture of the kidney, as revealed by histological examinations. Conclusions: According to the findings of this study, FRFAS provides dual protection to diabetic kidneys via antioxidant and anti-inflammatory properties, implying that FRFAS might be used as an adjuvant medication for the prevention and treatment of DN.