Kidney disease is one the secondary complications of diabetes. Probiotics are live microorganisms promoted with claims that they provide health benefits when consumed adequate amount, health benefits including antidiabetic, anti-inflammatory and antioxidant properties. This study aimed to determine the effects of probiotic supplementation on renal function and oxidative stress biomarkers in Type II Diabetes mellitus (T2DM) patients. In a randomized double-blind placebo-controlled clinical trial, 60 Subjects with aged 30–65 years were selected from CTR, Jiwaji University and assigned into two groups; Subjects in the probiotic group received a daily capsule containing Lactobacillus acidophilus, Bifidobacterium bifidium & Saccharomyces boulardii each 0.5 Bn/g and Lactobacillus plantarum, Bacillus clausii   each 0.25 bn/g with 125mg FOS. BD for 12 weeks. (Placebo group only 125mg FOS in a capsule). In the baseline and at the end of the study, physical activity levels, and dietary intakes were assessed. Anthropometric parameters, blood glucouse, creatinine, urea, BUN , uric acid  malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) activities were measured.Statistical analysis was carried out using a paired t-test and student t- test. There was no significant difference between the two groups for demographic characteristics, anthropometric parameters at the baseline of study.  The mean fasting blood glucose levels were reduced by 16.3 %.The probiotic supplementation resulted in a significant  improvement in Urea by  14.7 , 13.9%  BUN  14.2, 10.5% Uric acid  11.8, 8.9 %  Creatinine 11 ,6%   in subjects taken probiotic placebo capsules respectively. It also observed that the significant increase in GSH(p< 0.01), SOD(p< 0.01), CAT(p< 0.01) and decrease in MDA level (p< 0.01). Significant difference was observed between-group for these enzymes activities & other parameters at the end of the study. Overall, the results demonstrate that probiotics could improve renal function and oxidative stress factors among T2DM patients.