Introduction Focal segmental glomerulosclerosis (FSGS) is a syndrome that arises after podocyte injury from diverse causes. To identify causes of FSGS, previous studies showed that some podocyte-specific gene mutations were associated with primary FSGS. In this study, we investigated the gene linked with familial FSGS through whole exome sequencing (WES) of father and his son.   Case A 65 year old man was admitted for peripheral pitting edema with proteinuria over 10 years. His son was also diagnosed with FSGS and on HD treatment. The Laboratory test showed normal creatinine (0.8 mg/dL), elevated total cholesterol (263 mg/dL), lower normal serum albumin level (3.6 mg/dL) and protein 4+ in urinalysis. The patient underwent renal biopsy, and FSGS was identified. DNA was isolated from blood samples from patient, his wife, and his son using the DNA purification kit according to the manufacturer’s protocol. Whole exome sequencing was performed on the three family members. The obtained sequences of patient and his son were aligned to the reference genome of patient’s wife, and single-nucleotide polymorphisms were detected.   Result The patient and his son carried heterozygous mutations of CRB2 in exon 10 on chromosome 9 and TNS2 in exon 1 on chromosome 12. WES showed that c.C3329>T (predicting p.T1110M) in CRB2 and c.C38>T (predicting p.A13V) in TNS2 gean were inherited to his son. The CRB2 protein regulates podocyte cell polarity, and its heterozygous mutation was carried in FSGS patient. Although TNS2 function is unclear, its mutation is associated with murine podocyte foot process and 6 different mutations have been detected in families with nephrotic syndrome.   Conclusion We reported the case of FSGS patient carrying 2 heterozygous mutations, and genetic test using WES revealed that these mutations located in CRB2 and TNS2 gene inherited through parental line.